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<section id="phasorpy-homepage">
<h1>PhasorPy homepage<a class="headerlink" href="#phasorpy-homepage" title="Link to this heading">#</a></h1>
<p>Welcome to the homepage of the PhasorPy project.</p>
<p>PhasorPy is an open-source Python library for the analysis of luminescence
lifetime and hyperspectral images using the phasor approach.</p>
<section id="documentation">
<h2>Documentation<a class="headerlink" href="#documentation" title="Link to this heading">#</a></h2>
<p>The <a class="reference external" href="docs/stable/">PhasorPy documentation</a> thoroughly documents all aspects
of the library, including:</p>
<ul class="simple">
<li><p><a class="reference external" href="docs/stable/phasor_approach/">Phasor approach</a></p></li>
<li><p><a class="reference external" href="docs/stable/tutorials/">Tutorials</a></p></li>
<li><p><a class="reference external" href="docs/stable/api/">API reference</a></p></li>
<li><p><a class="reference external" href="docs/stable/release/">Release notes</a></p></li>
<li><p><a class="reference external" href="docs/stable/contributing/">Contributing guide</a></p></li>
<li><p><a class="reference external" href="docs/stable/acknowledgments/">Acknowledgments</a></p></li>
<li><p><a class="reference external" href="docs/stable/license/">MIT License</a></p></li>
</ul>
<p>Other versions: <a class="reference external" href="docs/dev">latest development</a>, <a class="reference internal" href="docs/"><span class="doc">all</span></a></p>
</section>
<section id="resources">
<h2>Resources<a class="headerlink" href="#resources" title="Link to this heading">#</a></h2>
<ul class="simple">
<li><p><a class="reference external" href="https://github.com/phasorpy/phasorpy">Source code</a> is maintained on GitHub.com.</p></li>
<li><p><a class="reference external" href="https://pypi.org/project/phasorpy/">Releases</a> are published on PyPI.org and <a class="reference external" href="https://anaconda.org/conda-forge/phasorpy">conda-forge</a>.</p></li>
<li><p><a class="reference external" href="https://zenodo.org/communities/phasorpy">Data files</a> are shared on Zenodo.org.</p></li>
<li><p><a class="reference external" href="https://github.com/phasorpy/phasorpy/issues">Ask questions and report issues</a> on the GitHub issue tracker.</p></li>
</ul>
</section>
<section id="news">
<h2>News<a class="headerlink" href="#news" title="Link to this heading">#</a></h2>
<ul class="simple">
<li><p>Jan 1, 2026: <a class="reference external" href="https://pypi.org/project/phasorpy/">PhasorPy 0.9</a> is released to PyPI.</p></li>
<li><p>Oct 10, 2025: <a class="reference external" href="https://pypi.org/project/phasorpy/">PhasorPy 0.8</a> is released to PyPI.</p></li>
<li><p>Aug 22, 2025: <a class="reference external" href="https://pypi.org/project/phasorpy/">PhasorPy 0.7</a> is released to PyPI.</p></li>
<li><p>Aug 16, 2025: The new <a class="reference external" href="https://www.phasorpy.org/_static/phasorpy_logo.svg">PhasorPy logo</a> is live.</p></li>
<li><p>June 22, 2025: <a class="reference external" href="https://pypi.org/project/phasorpy/">PhasorPy 0.6</a> is released to PyPI.</p></li>
<li><p>June 16, 2025: <a class="reference external" href="https://github.com/phasorpy/phasorpy/graphs/contributors">200th commit</a> to the PhasorPy source code.</p></li>
<li><p>Apr 11, 2025: <a class="reference external" href="https://pypi.org/project/phasorpy/">PhasorPy 0.5</a> is released to PyPI.</p></li>
<li><p>Jan 30, 2025: <a class="reference external" href="https://pypi.org/project/phasorpy/">PhasorPy 0.4</a> is released to PyPI.</p></li>
<li><p>Jan 25, 2025: The <a class="reference external" href="https://github.com/cgohlke/liffile">liffile library</a> for reading Leica LIF files is released.</p></li>
<li><p>Jan 8, 2025: PhasorPy is available on <a class="reference external" href="https://anaconda.org/conda-forge/phasorpy/">conda-forge</a>.</p></li>
<li><p>Dec 16, 2024: <a class="reference external" href="https://pypi.org/project/phasorpy/">PhasorPy 0.3</a> is released to PyPI.</p></li>
<li><p>Nov 30, 2024: <a class="reference external" href="https://pypi.org/project/phasorpy/">PhasorPy 0.2</a> is released to PyPI.</p></li>
<li><p>Oct 22, 2024: PhasorPy project update and practical are presented at the <a class="reference external" href="https://www.lfd.uci.edu/workshop/">18th LFD Workshop</a>.</p></li>
<li><p>Sept 30, 2024: <a class="reference external" href="https://pypi.org/project/phasorpy/">PhasorPy 0.1</a> is released to PyPI.</p></li>
<li><p>Sept 23, 2024: <a class="reference external" href="https://github.com/phasorpy/phasorpy/graphs/contributors">100th commit</a> to the PhasorPy source code.</p></li>
<li><p>June 12, 2024: PhasorPy is featured at the <a class="reference external" href="https://chanzuckerberg.com/science/programs-resources/open-science/">CZI Open Science 2024</a> meeting.</p></li>
<li><p>Feb 16, 2024: First <a class="reference external" href="https://www.phasorpy.org/docs/stable/tutorials/">tutorial</a> added to the documentation.</p></li>
<li><p>Nov 1, 2023: The <a class="reference external" href="https://github.com/cgohlke/ptufile">ptufile library</a> for reading PicoQuant PTU files is released.</p></li>
<li><p>Oct 26, 2023: The PhasorPy project is introduced at the <a class="reference external" href="https://www.lfd.uci.edu/workshop/2023/">17th LFD Workshop</a>.</p></li>
<li><p>Oct 5, 2023: The PhasorPy <a class="reference external" href="https://zenodo.org/communities/phasorpy">community on Zenodo</a> is used to share data files.</p></li>
<li><p>Sept 1, 2023: <a class="reference external" href="https://github.com/bruno-pannunzio">Bruno Pannunzio</a> joins the PhasorPy project.</p></li>
<li><p>Aug 22, 2023: The library documentation is published at <a class="reference external" href="https://www.phasorpy.org/docs/stable">www.phasorpy.org</a>.</p></li>
<li><p>Aug 21, 2023: First PhasorPy developer meeting, to be held remotely every two weeks.</p></li>
<li><p>July 6, 2023: PhasorPy’s <a class="reference external" href="https://github.com/phasorpy/phasorpy">organization and repository on GitHub</a> are live.</p></li>
<li><p>July 1, 2023: <a class="reference external" href="https://github.com/cgohlke">Christoph Gohlke</a> joins the PhasorPy project.</p></li>
<li><p>Sept 2022: A <a class="reference external" href="https://chanzuckerberg.com/eoss/proposals/phasorpy-a-python-library-for-phasor-analysis-of-flim-and-spectral-imaging/">grant from the Chan Zuckerberg Initiative</a>
is awarded to Leonel Malacrida and Enrico Gratton for the development of the PhasorPy library and community.</p></li>
</ul>
</section>
<section id="events">
<h2>Events<a class="headerlink" href="#events" title="Link to this heading">#</a></h2>
<p>The PhasorPy project is presented at the following events:</p>
<ul class="simple">
<li><p>Bruno Schüty.
PhasorPy hands-on training.
<a class="reference external" href="https://sites.uci.edu/aimlfdworkshop/">LFD/AIM Workshop on Fluorescence Advanced Imaging Research (FLAIR)</a>.
January 12-15, 2026. University of California, Irvine, USA.</p></li>
<li><p>Bruno Pannunzio and Leonel Malacrida.
The PhasorPy library for FLIM and HSI phasor analysis.
<a class="reference external" href="https://pasteur.uy/cursos-y-charlas/6th-workshop-in-advanced-microscopy-and-biophotonics/">6th Workshop in Advanced Microscopy and Biophotonics</a>.
November 23-28, 2025. Institut Pasteur de Montevideo, Uruguay.</p></li>
<li><p>Leonel Malacrida.
PhasorPy library for open-source phasor analysis of FLIM and HSI: present, future and some applications in biological fluorescence.
<a class="reference external" href="https://www.webersymposium.com/">12th International Weber Symposium</a>.
June 15-20, 2025. Genoa, Italy.</p></li>
<li><p>Bruno Pannunzio, Bruno Schuty Teske, Michelle Digman, Christoph Gohlke, and Leonel Malacrida.
PhasorPy: an open-source Python library for the analysis of fluorescence lifetime and hyperspectral images using the phasor approach.
<a class="reference external" href="https://biophysics.cld.bz/BPS2025-Full-Program-Abstracts/125/">69th Annual Meeting of the Biophysical Society</a>.
February 15-19, 2025. Los Angeles, USA.</p></li>
<li><p>Leonel Malacrida.
Introduction to PhasorPy.
Third LFD around the world meeting.
December 9, 2024. Online.</p></li>
<li><p>Leonel Malacrida.
The PhasorPy library for FLIM and HSI phasor analysis.
<a class="reference external" href="https://www.optica.org/events/webinar/2024/11_november/the_phasorpy_library_for_flim_and_hsi_phasor_analysis/">Optica Webinar Series</a>.
November 25, 2024. Online.
<a class="reference external" href="https://www.optica.org/events/webinar/2024/11_november/the_phasorpy_library_for_flim_and_hsi_phasor_analysis/">Recording</a>.</p></li>
<li><p>Bruno Schüty.
Phasor Analysis in fluorescence lifetime imaging and spectral imaging with PhasorPy.
<a class="reference external" href="https://www.globias.org/activities/past-activties/annual-workshop-gothenburg-2024">GloBIAS Bioimage Analysts Workshop</a>.
November 5-8, 2024. Gothenburg, Sweden.
<a class="reference external" href="https://www.youtube.com/watch?v=5qURI2NVkzg/">Recording</a>.</p></li>
<li><p>Bruno Pannunzio.
PhasorPy: an open-source Python library for the analysis of fluorescence lifetime and hyperspectral images using the phasor approach.
<a class="reference external" href="https://www.i2kconference.org/workshops">Virtual I2K 2024: Online tutorials on image analysis</a>.
October 29, 2024. Online.
<a class="reference external" href="https://www.youtube.com/watch?v=VGKGF8Zj3tY">Recording</a>.</p></li>
<li><p>Leonel Malacrida.
Unlocking the phasor approach for FLIM and HSI: Insights from PhasorPy, a comprehensive Python library for phasor plot analysis.
<a class="reference external" href="https://www.eurobioimaging.eu/events/phasor-plots-for-hyperspectral-imaging-and-flim/">Euro-Bioimaging Virtual Pub</a>.
October 25, 2024. Online.
<a class="reference external" href="https://www.youtube.com/watch?v=cI7WydgIG00">Recording</a>.</p></li>
<li><p>Bruno Schüty.
PhasorPy hands-on training.
<a class="reference external" href="https://www.lfd.uci.edu/workshop/">18th LFD Workshop in Advanced Fluorescence Imaging and Dynamics</a>.
October 21-24, 2024.
Laboratory for Fluorescence Dynamics, University of California, Irvine, USA.</p></li>
<li><p>Christoph Gohlke.
Introduction to PhasorPy.
<a class="reference external" href="https://www.lfd.uci.edu/workshop/">18th LFD Workshop in Advanced Fluorescence Imaging and Dynamics</a>.
October 21-24, 2024.
Laboratory for Fluorescence Dynamics, University of California, Irvine, USA.</p></li>
<li><p>Bruno Pannunzio, Bruno Schüty, Christoph Gohlke, and Leonel Malacrida.
PhasorPy: un nuevo software abierto para el análisis de imágenes espectrales y tiempo de vida por gráficos de fasores.
<a class="reference external" href="https://www.semanacademica.hc.edu.uy/index.php/galeria2024/681">Semana Académica del Hospital de Clínicas</a>.
September 23-27, 2024. Montevideo, Uruguay.</p></li>
<li><p>Leonel Malacrida.
Phasor analysis for FLIM & STED.
<a class="reference external" href="https://www.canadabioimaging.org/mlmc-infocus-sted">Montreal Light Microscopy Course (MLMC). In-Focus: STimulated Emission Depletion (STED) Microscopy</a>.
July 22-26, 2024. Montreal, Canada.</p></li>
<li><p>Leonel Malacrida.
The phasor approach for biophysical studies of condensates and membranes using DAN probes.
<a class="reference external" href="https://www.sibpa.it/CongressoNazionaleSIBPAGenova/">XXVII Congreso Nazionale SIBPA</a>.
June 16-20, 2024. Genoa, Italy.</p></li>
<li><p>Leonel Malacrida.
PhasorPy: a Python open-source library for phasor analysis of FLIM and HSI data.
<a class="reference external" href="https://chanzuckerberg.com/science/programs-resources/open-science/">CZI Open Science 2024</a>.
June 11-14, 2024. Boston, USA.</p></li>
<li><p>Leonel Malacrida.
The phasor plots for time-resolved and hyperspectral imaging: fundamentals and new developments.
<a class="reference external" href="https://www.samictucuman2024.com/">Sociedad Argentina de Microscopia</a>.
May 29-31, 2024. Tucuman, Argentina.</p></li>
<li><p>Leonel Malacrida.
The phasor plot analysis for hyperspectral imaging and time-resolved imaging: PhasorPy developments and its applications.
<a class="reference external" href="https://librehub.github.io/2024/04/03/leonel-malacrida.html">Latin American Hub for Bioimaging Through Open Hardware (LIBRE Hub) Seminars</a>.
May 15, 2024. Online.
<a class="reference external" href="https://www.youtube.com/watch?v=CbmDNjwo_sg">Recording</a>.</p></li>
<li><p>Leonel Malacrida.
PhasorPy: a Python open-source library as SimFCS legacy for phasor analysis of FLIM and HSI data.
<a class="reference external" href="https://www.lfd.uci.edu/frontiers/">Frontiers in Biological Fluorescence 2024</a>.
May 10, 2024. University of California, Irvine, USA.</p></li>
<li><p>Leonel Malacrida.
Microscopía no lineal en Uruguay: 2P-FLIM y microscopio DIVER para estudiar procesos in vivo en la profundidad del tejido.
<a class="reference external" href="https://cicada.uy/simposio-en-microscopia-y-bioimagenes-avances-y-desafios/">Simposio en Microscopía y Bioimágenes: avances y desafíos</a>.
April 4-5, 2024. University of the Republic (UdelaR). Montevideo, Uruguay.</p></li>
<li><p>Leonel Malacrida.
Microscopy techniques applied to the analysis of biosensors performance.
<a class="reference external" href="https://pasteur.uy/cursos-y-charlas/1st-latin-american-workshop-development-and-applications-of-biosensors-from-fluorescent-proteins-to-synthetic-biology/">1st Latin-American Workshop: Development and applications of biosensors: from fluorescent proteins to synthetic biology</a>.
Nov 27 - Dec 1, 2023. Institut Pasteur de Montevideo, Uruguay.</p></li>
<li><p>Bruno Schüty, Bruno Pannunzio, and Leonel Malacrida.
Practical section 3: PhasorPy and Napari plugin for FLIM & HSI analysis.
<a class="reference external" href="https://pasteur.uy/2023/5th-workshop-in-advanced-microscopy-and-biophotonics/">5th Workshop in Advanced Microscopy and Biophotonics</a>.
November 19-24, 2023. Institut Pasteur de Montevideo, Uruguay.</p></li>
<li><p>Christoph Gohlke and Leonel Malacrida.
Introduction to PhasorPy.
<a class="reference external" href="https://www.lfd.uci.edu/workshop/">17th LFD Workshop in Advanced Fluorescence Imaging and Dynamics</a>.
October 23-27, 2023. Laboratory for Fluorescence Dynamics,
University of California, Irvine, USA.</p></li>
</ul>
</section>
<section id="cite">
<h2>Cite<a class="headerlink" href="#cite" title="Link to this heading">#</a></h2>
<p>Please cite <a class="reference external" href="https://dx.doi.org/10.5281/zenodo.13862586">doi: 10.5281/zenodo.13862586</a>
if PhasorPy contributes to research that leads to a publication.</p>
</section>
<section id="contact">
<h2>Contact<a class="headerlink" href="#contact" title="Link to this heading">#</a></h2>
<p>PhasorPy is a community-maintained project.</p>
<p><a class="reference external" href="docs/stable/contributing/">Contributions</a>
in the form of bug reports, bug fixes, feature implementations, documentation,
datasets, and enhancement proposals are welcome.</p>
<p>Report issues and ask questions about PhasorPy on the GitHub
<a class="reference external" href="https://github.com/phasorpy/phasorpy/issues">issue tracker</a>.</p>
<p>Alternatively, contact the
<a class="reference external" href="https://github.com/orgs/phasorpy/people">PhasorPy developers</a> directly.</p>
</section>
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